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KMID : 1145120160140030277
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Asian Journal of Beauty and Cosmetology
2016 Volume.14 No. 3 p.277 ~ p.288
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A Study on the Utilization of Dendropanax morbifera Lev. Leaf Extract for Material of Functional Cosmetics and Hair Growth Products
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Park Yun-Mi
Han Jin-Seop
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Abstract
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Purpose: This study was conducted to provide data of the material development of functional cosmetics and hair growth products that could replace the drug therapies by extracting the active ingredients from Dendropanax morbifera (D. morbifera) leaf using the methanol-ultrasound extraction method.
Methods: Total polyphenol and flavonoid contents of D. morbifera leaf methanol-ultrasound extract were measured. Also, the anti-oxidant capacity, nitrite scavenging ability, anti-microbial activity, and the minimum inhibitory concentration (MIC) were measured. Cytotoxicity of the extract on HFDPCs was examined by MTT assay at concentrations of 10, 100, and 500 μg/mL, respectively. Moreover, the promotive and inhibitory effects of growth factor expression were also confirmed through the western blotting.
Results: Total polyphenol and flavonoid contents of D. morbifera leaf methanol-ultrasound extract were 74.082±7.180 mg/g and 97.360±2.235 mg/g, respectively. DPPH scavenging ability was 92.440±1.023%, which was very high, and the nitrite scavenging activity was 52.6±2.4%. Anti-microbial activities were investigated in the strains such as Propionibacterium acnes (P. acnes), Pityrosporum ovale (P. ovale), and Malassezia furfur (M. furfur). The MIC of P. acnes, P. ovale, and M. furfur were 17.3%, 21.6%, and 15.7%, respectively. Cytotoxicity was not observed within the range of concentrations (10, 100, and 500 μg/mL) of D. morbifera leaf methanol-ultrasound extract and the cell growth rate was increased according to the concentrations. In case of the growth factor expression by western blotting, high promotive effect was observed in IGF protein expression (156±8%) compared with the control group, while inhibitory effect was confirmed in TGFβ2 protein expression (78.3±10%).
Conclusion: Therefore, it is possible to utilize D. morbifera leaf methanol-ultrasound extract as the material of functional cosmetics and hair growth products with anti-oxidant capacity.
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KEYWORD
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Dendropanax morbifera, Human hair follicle dermal papilla cell, Western blotting, Growth factor protein, DPPH
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